Furthermore, SAGA and SWI/SNF were both found to specifically organize the chromatin structure at the arsenic response locus for activation of gene transcription. Functional assays identified the histone acetyltransferase SAGA and the chromatin remodelling complex SWI/SNF to be required for activation of the locus. CRISPR-ChAP-MS was applied to the promoter regions of the activated arsenic response locus and uncovered 40 nuclear-annotated proteins showing enrichment. An affinity enrichment strategy called CRISPR-Chromatin Affinity Purification with Mass Spectrometry (CRISPR-ChAP-MS) was used, which provides for the proteomic characterization of a targeted locus. identify how transcription from the arsenic response locus is regulated in an arsenic dependent manner. This locus constitutes a conserved pathway ranging from prokaryotes to higher eukaryotes. The arsenic response locus in budding yeast is responsible for the detoxification of arsenic and its removal from the cell.
Millions of people encounter arsenic through contaminated drinking water, consumption, and inhalation. Arsenic exposure is a global health problem.
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